Evaluation of Neuroprotective Activity of Chrysin Nanoparticles Using Scopalamine Induced Neuroinflammation in the Rat Model of Alzheimer’s Disease

Abstract

Background: scopolamine induced neuroinflammation limits its long-term clinical use. Several agents/strategies were attempted to prevent scopolamine neuroinflammatory but were not found suitable for clinical use. Objective: To investigate the neuroprotective activity of chrysin nanoparticle on scopolamine induced neuroinflammation. Material and Method: Male Albino wistarrats (180- 230 grms) were evenly divided into 5 groups. Group I&II served as control (distilled  water  10ml/kg) and diseases control (scopolamine) 100mg/kg per os treated for 4 weeks, group III served as standard group which received Donepezil (1.5mg/kg) on ,while group IV and V are simultaneously treated with (50mg/kg and 100 mg/kg) orally with chrysin nanoparticle up to 4 weeks. On 29 and 30 day behavioral parameters and brain were isolated for biochemical parameters at the same time rat brains were used for histological studies. Results: Treatment of scopolamine caused neuroinflammation as the evidence by marked elevation in AchE, GSH, catalase, super oxide Dismutase, Elevated plus maze,Y-maze and Open field test. SLN chrysin decrease in AchE, catalase, super oxide dismutase, GSH time respectively. SLN chrysin increase activities of SOD, crossing, Rearing, Grooming. Histopathological changes also showed the protective nature SLN chrysin induced neuroinflammation