Computer aided analysis of antigen-antibody reactions

ACM-SE 14 Pub Date : 1976-04-22 DOI:10.1145/503561.503601
B. Liu, J. Steensgaard
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引用次数: 1

Abstract

Antibodies are large molecules which are formed in the body to render antigens (causes of diseases) harmless. Bivalent antibodies and multivalent antigens may form a rich variety of different antigen-antibody complexes. The outcome of a reaction between an antigen and its antibodies can be analyzed experimentally by use of a gradient centrifugation technique.We have used computer models to study the extent to which the outcome of an antigen-antibody reaction is predictable from simple assumptions.First, a mathematical model for simulation of this particular centrifugal technique was constructed, allowing calculation of where in the centrifuge rotor a given complex would be located following centrifugation under precisely defined circumstances. Second, a program to predict the outcome of reactions between antigens and antibodies was written, based on the assumption that the kinds and the amounts of antigen-antibody complexes are determined by the concentrations of antigens and antibodies, respectively, together with a parameter describing their mutual affinity. Third, these programs were combined so as to calculate the expected outcome of antigen-antibody reactions as they appear after this type of centrifugal analysis. Finally, calculated results were compared to experimentally obtained results, and a fairly good agreement was found.It is concluded that the main characteristical features of such reactions are predictable from the above mentioned assumptions by use of these computer models.
抗原-抗体反应的计算机辅助分析
抗体是在体内形成的大分子,使抗原(疾病的原因)无害。二价抗体和多价抗原可形成丰富多样的不同抗原-抗体复合物。抗原和抗体反应的结果可以用梯度离心技术进行实验分析。我们已经使用计算机模型来研究抗原-抗体反应的结果在多大程度上可以通过简单的假设来预测。首先,建立了模拟这种特殊离心技术的数学模型,允许在精确定义的情况下计算离心后给定复合物在离心机转子中的位置。其次,基于抗原-抗体复合物的种类和数量分别由抗原和抗体的浓度以及描述它们相互亲和力的参数决定的假设,编写了一个预测抗原和抗体之间反应结果的程序。第三,将这些程序结合起来,计算出这种离心分析后出现的抗原-抗体反应的预期结果。最后,将计算结果与实验结果进行了比较,两者吻合较好。结论是,利用这些计算机模型可以根据上述假设预测这些反应的主要特征。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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