Cesalin--an anti-neoplastic protein.

Lloydia Pub Date : 1977-05-01
R Montgomery, F Yamauchi
{"title":"Cesalin--an anti-neoplastic protein.","authors":"R Montgomery,&nbsp;F Yamauchi","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>An anti-neoplastic protein was isolated from the endosperm of the seeds of Caesalpinia gilliesii by extraction with water, dialysis and precipitation by ammonium sulfate or acidification. The precipitated protein mixture was separated by column chromatography into three principal proteins, one of which, termed cesalin, inhibited the growth of Walker 256 carcinosarcoma. There is an associated carbohydrate with the cesalin that can be largely removed by chromatography on hydroxyl apatite; the remaining carbohydrate (about 0.3%) is a hexosan. Cesalin, molecular weight 110,000, migrates as a single component by polyacrylamide gel electrophoresis at pH 8.3, but in a denaturing system containing sodium dodecyl sulfate three bands were observed. The correspond to protein sub-units of approximately 30,000 daltons. Anti-tumor tests in rats showed 70-80% inhibition of Walker 256 growth at a dose of 80 microgram/kg/day of cesalin.</p>","PeriodicalId":18256,"journal":{"name":"Lloydia","volume":"40 3","pages":"269-74"},"PeriodicalIF":0.0000,"publicationDate":"1977-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lloydia","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

An anti-neoplastic protein was isolated from the endosperm of the seeds of Caesalpinia gilliesii by extraction with water, dialysis and precipitation by ammonium sulfate or acidification. The precipitated protein mixture was separated by column chromatography into three principal proteins, one of which, termed cesalin, inhibited the growth of Walker 256 carcinosarcoma. There is an associated carbohydrate with the cesalin that can be largely removed by chromatography on hydroxyl apatite; the remaining carbohydrate (about 0.3%) is a hexosan. Cesalin, molecular weight 110,000, migrates as a single component by polyacrylamide gel electrophoresis at pH 8.3, but in a denaturing system containing sodium dodecyl sulfate three bands were observed. The correspond to protein sub-units of approximately 30,000 daltons. Anti-tumor tests in rats showed 70-80% inhibition of Walker 256 growth at a dose of 80 microgram/kg/day of cesalin.

凯撒林——一种抗肿瘤蛋白。
采用水提、透析、硫酸铵沉淀或酸化等方法,从沙麻种子胚乳中分离到一种抗肿瘤蛋白。沉淀的蛋白质混合物通过柱层析分离成三种主要蛋白质,其中一种称为cesalin,可以抑制Walker 256癌肉瘤的生长。有一种与凯撒林相关的碳水化合物,可以通过羟基磷灰石层析除去;剩下的碳水化合物(约0.3%)是己糖。聚丙烯酰胺凝胶电泳显示,分子量为11万的赛沙林在pH 8.3条件下以单一组分迁移,但在含十二烷基硫酸钠的变性体系中观察到三条条带。这相当于蛋白质亚单位约30,000道尔顿。大鼠抗肿瘤试验显示,在80微克/公斤/天的剂量下,cesalin对Walker 256的生长有70-80%的抑制作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信