Controllable protein network based on DNA-origami and biomedical applications

Abstract

As an important part of driving natural life systems, the function of protein networks is accurately controlled through many parameters, like distance, quantity, position, and orientation. Nevertheless, it would be very hard to control the physical arrangement of the multiple proteins to generate cellular signaling events or complex enzymatic cascades, for instance small molecule organic synthesis DNA nanotechnology provides matching nanoscale dimensions, the special programmability of DNA, and the capability and compatibility of many proteins and nucleic acids. DNA origami has precise addressing capabilities at the nanoscale, which ensures the accurate assembly of the protein networks. These characteristics indicate that the DNA origami is a highly addressable programmable nanomaterial, which can be applied for building artificial protein networks. Up to now, researchers have achieved significant progress in the establishment and application of the DNA origami-protein networks. In the current review, we introduce the superiorities of DNA origami-protein networks in detail, concluded their construction strategies, and their recent progression and applications in biomedicine and biophysics. In the end, we look into the future prospects of DNA origami-protein networks. Finally, we looked forward to the future perspective of DNA origami-protein networks.