Determination of epimeric 1-(3-indolyl) propane -1,2,3-triol isolated from Balansia epichloe.

Abstract

Investigations into diseases in cattle grazing on grass pastures infected with clavicipitaceous fungi have resulted in the isolation and characterization of erythro and threo 1-(3-indolyl) propane-1,2,3-triol from cultures of Balansia epichloë (Weese). Gas chromatography-mass spectroscopy analyses of synthetic and natural epimers as their trimethylsilyl derivatives is described. Toxicity studies with fertile chicken eggs demonstrated that the threo epimer was the more active compound. The ratio of erythro to threo was calculated at 3:2 in the natural isolate.