Identification and fine mapping of PmNJ3946 for powdery mildew resistance in einkorn wheat

IF 6 1区 农林科学 Q1 AGRONOMY
Peisi Wang , Jun Huang , Na Li , Jie Zhang , Caimei Gu , Yang Yuan , Ziruo Wen , Haiyan Jia , Zhongxin Kong , Zhengqiang Ma
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引用次数: 0

Abstract

Powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is a destructive wheat disease. Although it can be easily overcome by deployment of resistance genes, the resistance is often quickly compromised by pathogen virulence. Thus, exploration and characterization of new resistance genes is always ongoing. Line NJ3946 derived from a cross of einkorn wheat accessions TA2032 and M389 showed resistance to powdery mildew. Inheritance analysis of an F2 population derived from a cross of NJ3946 and M389 suggested that the resistance was conferred by a dominant allele. With polymorphic markers identified through bulked segregant analysis (BSA), this gene was mapped to a novel locus on chromosome 3A, and was designated as PmNJ3946. Bulked segregant RNA-seq analysis (BSR-seq) was conducted to obtain more closely linked markers, which allowed delimitation of the PMNJ3946 locus to a 0.9 cM interval covering a physical distance of less than 1 Mb. PMNJ3946 was flanked by Xwgrc5153 and SNP-derived marker CHS21_3A008915069, and co-segregated with SNP-derived markers CHS21_3A008939814 and CHS21_3A008943175. The PmNJ3946 discovery expands the diversity of powdery mildew resistance genes and is useful for wheat breeding.

小麦白粉病抗性 PmNJ3946 的鉴定和精细图谱绘制
由 Blumeria graminis f. sp. tritici(Bgt)引起的白粉病是一种毁灭性的小麦病害。虽然抗性基因的应用可以很容易地克服这种病害,但抗性往往很快就会被病原菌的毒力所破坏。因此,对新抗性基因的探索和鉴定一直在进行中。由银杏小麦品种 TA2032 和 M389 杂交产生的品系 NJ3946 显示出对白粉病的抗性。对 NJ3946 和 M389 杂交产生的 F2 群体的遗传分析表明,抗性是由显性等位基因赋予的。通过批量分离分析(BSA)确定的多态性标记,该基因被映射到 3A 染色体上的一个新基因座上,并被命名为 PmNJ3946。为了获得更多紧密相连的标记,进行了大量分离 RNA-seq 分析(BSR-seq),从而将 PMNJ3946 基因座划定在物理距离小于 1 Mb 的 0.9 cM 区间。PMNJ3946 位于 Xwgrc5153 和 SNP 衍生标记 CHS21_3A008915069 的侧翼,并与 SNP 衍生标记 CHS21_3A008939814 和 CHS21_3A008943175 共分离。PmNJ3946 的发现扩大了白粉病抗性基因的多样性,有助于小麦育种。
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来源期刊
Crop Journal
Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍: The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.
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