The Interaction of Tungsten Dust with Human Skin Cells

Lavinia Gabriela Carpen, T. Acsente, Maria Adriana Acasandrei, E. Matei, Claudia Gabriela Chilom, Diana Iulia Savu, G. Dinescu
{"title":"The Interaction of Tungsten Dust with Human Skin Cells","authors":"Lavinia Gabriela Carpen, T. Acsente, Maria Adriana Acasandrei, E. Matei, Claudia Gabriela Chilom, Diana Iulia Savu, G. Dinescu","doi":"10.5772/intechopen.86632","DOIUrl":null,"url":null,"abstract":"In this chapter, we evaluate the tungsten (W) nanoparticle toxicity with respect to the normal human skin fibroblast cell. Tungsten dust formation is expected in the tokamak-type nuclear fusion installations, regarded as future devices for large-scale, sustainable, and carbon-free energy. This dust, composed of tungsten particles of variable size, from nanometers to micrometers, could be harmful to humans in the case of loss of vacuum accident (LOVA). In order to undertake the toxicity studies, tokamak-relevant dust has been deliberately produced in laboratory and afterward analyzed. Following that, cytotoxicity tests were performed using normal human skin fibroblast cell lines, BJ ATCC CRL 2522. Our study concludes that, at a low concentration (until 100 μ g/mL), no cytotoxic effect of tungsten nanoparticles was observed. In contrast, at higher concentrations (up to 2 mg/mL), nanometric dust presents toxic effects on the cells. nanoparticles that are internalized under the membrane. These latter nanoparticles can be observed through the backscattered electron technique.","PeriodicalId":421288,"journal":{"name":"Nanomaterials - Toxicity, Human Health and Environment","volume":"4 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2019-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanomaterials - Toxicity, Human Health and Environment","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5772/intechopen.86632","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3

Abstract

In this chapter, we evaluate the tungsten (W) nanoparticle toxicity with respect to the normal human skin fibroblast cell. Tungsten dust formation is expected in the tokamak-type nuclear fusion installations, regarded as future devices for large-scale, sustainable, and carbon-free energy. This dust, composed of tungsten particles of variable size, from nanometers to micrometers, could be harmful to humans in the case of loss of vacuum accident (LOVA). In order to undertake the toxicity studies, tokamak-relevant dust has been deliberately produced in laboratory and afterward analyzed. Following that, cytotoxicity tests were performed using normal human skin fibroblast cell lines, BJ ATCC CRL 2522. Our study concludes that, at a low concentration (until 100 μ g/mL), no cytotoxic effect of tungsten nanoparticles was observed. In contrast, at higher concentrations (up to 2 mg/mL), nanometric dust presents toxic effects on the cells. nanoparticles that are internalized under the membrane. These latter nanoparticles can be observed through the backscattered electron technique.
钨尘与人体皮肤细胞的相互作用
在本章中,我们评估了钨(W)纳米颗粒对正常人类皮肤成纤维细胞的毒性。预计在托卡马克型核聚变装置中会形成钨尘,该装置被视为未来大规模、可持续和无碳能源的装置。这种粉尘由不同尺寸的钨颗粒组成,从纳米到微米不等,在失去真空事故(LOVA)的情况下可能对人类有害。为了进行毒性研究,故意在实验室中产生与托卡马克有关的粉尘,然后进行分析。随后,使用正常人皮肤成纤维细胞系(BJ ATCC CRL 2522)进行细胞毒性试验。我们的研究表明,在低浓度(直到100 μ g/mL)下,钨纳米颗粒没有细胞毒性作用。相反,在更高的浓度下(高达2毫克/毫升),纳米粉尘对细胞有毒性作用。被内化在膜下的纳米颗粒。后一种纳米粒子可以通过背散射电子技术观察到。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信