Effectiveness Microcapsules From Auxin Producing-Endophite Bacteria As Biofertilizer in Tea (Camellia sinensis L)

Sigit Indra Zit, K. Warsito, Refnizuida Refnizuida
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Abstract

Tea (Camellia sinensis L), is a popular agricultural product enjoyed by people around the world. Bio-fertilizers that use bacteria found in soil called endophytes, have proven effective for increasing growth and plant productivity. Endophite bacteria can also produce growth hormones such as auxins, ethylene, and cytokinins. This research aims to determine the effectiveness of auxin-producing endophytic bacterial microcapsules on tea growth. The research design used was a completely randomized design (CRD), 16 treatments with 2 replications. The first factor was immersion of  endophite bacterial suspension consisting of S0 = 0 hours; S1 = 24 hours; S2 = 36 hours and S3 = 48 hours and the second factor was the addition of microcapsules consisting of I0 = 0 gr; I1 = 5 gr; I2 = 10 gr; I3 = 15 gr. Isolation from tea roots and stems obtained 6 isolates of endophite  bacteria. Auxin test showed that the six isolates were able to produce auxin. Observation of plant height showed that the best treatment was in treatment I3 (17.04 cm). Observation of leaves amount was in treatment I3 (6.88 strands). For the leaf area parameter, the highest number was in the S1 treatment (22.8 cm2). For stem diameter parameter, highest data was in treatment I2 (1.69 mm). The test results showed that the application of suspension and endophite bacteria microcapsules  significantly increased the growth of tea.
产生长素内生细菌微胶囊作为茶树生物肥料的有效性研究
茶(Camellia sinensis L)是一种深受世界人民喜爱的农产品。生物肥料利用土壤中被称为内生菌的细菌,已被证明对促进植物生长和生产力有效。内生细菌也能产生生长激素,如生长素、乙烯和细胞分裂素。本研究旨在研究生长素内生细菌微胶囊对茶叶生长的影响。采用完全随机设计(CRD), 16个处理,2个重复。第一个影响因素是内生菌悬浮液的浸泡时间为0 = 0小时;S1 = 24小时;S2 = 36 h, S3 = 48 h,第二个影响因素是添加由I0 = 0 gr组成的微胶囊;I1 = 5 gr;I2 = 10 gr;从茶根和茎中分离得到6株内生细菌。生长素试验表明,6株菌株均能产生生长素。株高观察结果显示,处理I3为最佳处理(17.04 cm)。叶片数量观察为处理I3(6.88株)。叶面积参数以S1处理最高(22.8 cm2);茎径参数以处理2最高(1.69 mm)。试验结果表明,施用悬浮液和内生细菌微胶囊显著促进了茶叶的生长。
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