{"title":"KELIMPAHAN BAKTERI PENDEGRADASI MINYAK BUMI DI PT. INDUSTRI KAPAL INDONESIA (Persero) MAKASSAR Makassar","authors":"Isnada Sulaiman","doi":"10.26858/jptp.v5i0.8558","DOIUrl":null,"url":null,"abstract":"This research is a descriptive study that aims to determine the abundance of marine bacteria at PT. Indonesian Ship Industry (Persero) in degrading petroleum. Water samples were taken from the territorial waters of PT. Indonesian Ship Industry (Persero) Makassar, with sampling points of 1 to 45.7 meters. Then diluted from 10-1 to 10-4, each dilution (10-1-10-4) was inoculated on a minimal solid medium using the pour cup method, the cup was incubated at 28ºC-30ºC for 48 hours. The data obtained are then analyzed using the Standard Plate Count (SPC) formula and then followed by observations of colonies in the form of colonies, color elevations, and internal structure. The results showed that the difference in bacterial abundance at the sampling point of 1 meter to 45.7 meters was the decrease in bacterial abundance from, taking point 3.6 meters to 45.7 meters while the observation of colonies took the form of edge elevation, color and structure in the colony. The results showed that there were colonies which were round, curly, filamentous, and irregular with convex and convex elevations, jagged edges, wavy, and even with a rough and smooth texture. The inner structure is clear, blurred and slippery and has a yellow and white color.","PeriodicalId":416159,"journal":{"name":"Jurnal Pendidikan Teknologi Pertanian","volume":"46 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jurnal Pendidikan Teknologi Pertanian","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.26858/jptp.v5i0.8558","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
This research is a descriptive study that aims to determine the abundance of marine bacteria at PT. Indonesian Ship Industry (Persero) in degrading petroleum. Water samples were taken from the territorial waters of PT. Indonesian Ship Industry (Persero) Makassar, with sampling points of 1 to 45.7 meters. Then diluted from 10-1 to 10-4, each dilution (10-1-10-4) was inoculated on a minimal solid medium using the pour cup method, the cup was incubated at 28ºC-30ºC for 48 hours. The data obtained are then analyzed using the Standard Plate Count (SPC) formula and then followed by observations of colonies in the form of colonies, color elevations, and internal structure. The results showed that the difference in bacterial abundance at the sampling point of 1 meter to 45.7 meters was the decrease in bacterial abundance from, taking point 3.6 meters to 45.7 meters while the observation of colonies took the form of edge elevation, color and structure in the colony. The results showed that there were colonies which were round, curly, filamentous, and irregular with convex and convex elevations, jagged edges, wavy, and even with a rough and smooth texture. The inner structure is clear, blurred and slippery and has a yellow and white color.
本研究是一项描述性研究,旨在确定PT. indonesia Ship Industry (Persero)降解石油中海洋细菌的丰度。水样取自印尼望加锡船业公司领海,采样点为1至45.7米。然后从10-1稀释至10-4,每次稀释(10-1-10-4)采用倒杯法接种于最小固体培养基上,在28ºC-30ºC条件下孵育48小时。然后使用标准平板计数(SPC)公式分析获得的数据,然后以菌落,颜色高度和内部结构的形式观察菌落。结果表明:1 m至45.7 m取样点细菌丰度差异为3.6 m至45.7 m取样点细菌丰度下降,菌落观察以菌落边缘高程、菌落颜色、菌落结构等形式进行。结果表明:菌落有圆形、卷曲、丝状、不规则等,有凹凸不平、边缘参差不齐、波浪状,甚至有粗糙光滑的纹理。内部结构清晰、模糊、光滑,颜色呈黄白色。