BIOSENSOR FOR ALT MEASUREMENT

Abstract

In this work, an amperometric biosensor to determine ALT, which will be part of a biosensor system for simultaneous measurement of AST and ALT and their ratios, was developed. A platinum disk electrode was used as the electrochemical transducer. To form a bioselective element, a mixture based on glutamate oxidase was applied to the surface of transducer. A universal method for measuring ALT activity using the developed biosensor was proposed and described. During the study, the optimal concentrations of ALT substrates (α-ketoglutarate - 50 μM, alanine - 4 mM) and coenzyme (50 mkM of PLP) were selected. The analytical characteristics of the developed biosensor were also investigated, namely, sensitivity, linear range, minimum limit of determination, selectivity, stability, etc. It was shown the fundamental possibility of using this biosensor to measure the content of ALT in real samples.