Isolation and Activation of Fresh Fruit Bunch Oil Palm Acetyl Co-A Carboxylase

Abstract

Palm oil has a substantial contribution to national economy, as well as local economy development. At present the productivity of Indonesian oil plam plantation, especially small holderd is relatively low. Efforts to increase productivity can be done through biochemicals approach in this case enzymes that responsible for oil production. One of the bottle neck for oil production is the step converting acetyl co-A to malonyl co-A by acetyl co-A carboxylase (ACCs). ACCs in human is known to be activated with certain amino acid and organic acids, and divalen metals. For this reason this study was conducted to separate and study the activation of ACCs by those substances. Activity of ACCs was measured developed using HPLC method. Oil palm ACCs had 50 kDa of Biotin Carboxylase (BC) subunit dan 31 kDa of Carboxyl Transferase (CT) subunit. Separation of crude extract ACCs using dialysis and sephadex G-25 column chromatography showed the higher activity of enzyme. Addition of activator in the form of citric acid, Mg 2+ and glutamic acid could stimulate activity of the enzyme.