Protective effect of Majoon Muṣaffī-i-Khūn (MMK) in cyclophosphamide-induced thrombocytopenia in male Sprague Dawley rats

Abstract

Aim of the study: To evaluate the efficacy of Majoon Muṣaffī-i-Khūn (MMK) in cyclophosphamide-induced thrombocytopenia in male Sprague Dawley rats. Material and Methods: Thrombocytopenia was induced by administration of Cyclophosphamide (25 mg/kg, 3 days, SC). Simultaneously, the animals were treated with MMK (300 and 600 mg/kg) orally and continued for three weeks. After completion of treatment, blood samples were collected by retro-orbital puncture and analyzed for WBC, RBC, Hb, and platelet counts, with bleeding and clotting time. MMK insignificantly increased the platelets, WBC, RBC, and Hb with a significant decreased in bleeding (p<0.001; p<0.05) and clotting times (P<0.01, P<0.001) which was altered by cyclophosphamide. Results: The results demonstrated that MMK improved platelet counts, clotting, and bleeding time as well as restored the altered level of WBC, RBC, and Hb. Conclusion: Based on the above results it can be concluded that the hydroalcoholic extract of MMK may have the potential to normalize the blood composition effectively.