In-vivo analysis of human cancer cells by means of laser phase microscopy

Abstract

Phase images of human colon carcinoma cells CX1, HCT15, HCT116 and human urinary bladder carcinoma cells EJ28 were obtained with laser phase microscope and have been analyzed. The phase shift images are compared with conventional light microscopy and confocal laser scanning microscopy data in order to detect the correspondence between morphological peculiarities and phase characteristics of measured cell. Influence of interactions between human carcinoma cells and specific antibodies (CC18 monoclonal antibody to cytokeratin and MRK 16 monoclonal antibody) on the cells phase images was studied.