Assessment of the Effect of Biopesticides ASTOUN 50 EC and NOSTAG 50 EC on Fusarium Wilt Pathogen (Fusarium sp.) and on Some Agro-morphological Parameters of Common Bean (Phaseolus vulgaris L.)

Kouamé Koffi Gaston, K. K. Didier, Coulibaly Lacina Fanlégué, Tuo Seydou, Dongo Roger Fulgence Kouassi, Yao Kouadio Jacques, Koné Daouda
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Abstract

Fusarium wilt caused by the fungus Fusarium sp. is rife in all common bean production areas in Côte d'Ivoire. It causes wilting and/or death of plants and yield losses. Chemical control is the most widely used method against this pathology. But the misuse of this method results in environmental pollution, pathogen resistance and human health problems. The search for an alternative solution to chemical control is necessary. It is in this context that this study was initiated. It aims at assessing in vitro and on-farm the effectiveness of the bio-pesticides ASTOUN 50 EC and NOSTAG 50 EC against common bean fusarium wilt pathogen. For this purpose, in vitro, five (5) doses (100, 200, 500, 1000 and 2000 µL/L) of each of the two biopesticides were tested by the method of incorporation into the PDA medium. Under natural conditions, the IC90 and MIC of each bio-pesticide were tested. The results showed that the two bio-pesticides completely inhibited the mycelial growth of Fusarium sp. at doses of 500 μl/L. NOSTAG 50 EC showed an IC90 at 300 μl/L and ASTOUN 50 EC at 400 μl/L. The biopesticides, on-farm, influenced the germination and growth parameters and reduced the wilting of the plants. In short, the 500 μl/L dose of the two biopesticides can be recommended in fusarium wilt control.
生物农药ASTOUN 50 EC和NOSTAG 50 EC对菜豆枯萎病病原菌及部分农业形态参数影响的评价
由真菌镰刀菌引起的枯萎病在Côte科特迪瓦所有普通豆类产区普遍存在。它导致植物枯萎和/或死亡,并造成产量损失。化学防治是对付这种病理最广泛使用的方法。但这种方法的滥用造成了环境污染、病原体耐药性和人体健康问题。寻找化学控制的替代方案是必要的。正是在这种背景下,这项研究开始了。本试验旨在评价ASTOUN 50ec和NOSTAG 50ec两种生物农药对普通豆枯萎病病原菌的体外和田间药效。为此,在体外,通过掺入PDA培养基的方法测试两种生物农药各5(5)个剂量(100、200、500、1000和2000µL/L)。在自然条件下,测定各生物农药的IC90和MIC。结果表明,两种生物农药在500 μl/L剂量下均能完全抑制镰刀菌菌丝的生长。NOSTAG 50 EC在300 μl/L和ASTOUN 50 EC在400 μl/L时的IC90均为最佳。在田间施用生物农药,影响了植物的发芽和生长参数,减少了植物的萎蔫。综上所述,两种生物农药在防治枯萎病时推荐用量为500 μl/L。
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