Overexpression of molecular chaperons GRP78 and GRP94 in CD44hi/CD24lo breast cancer stem cells

BioImpacts : BI Pub Date : 2016-06-30 DOI:10.15171/bi.2016.15

Babak Nami, Armin Ghasemi-Dizgah, A. Vaseghi

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引用次数: 26

Abstract

Introduction: Breast cancer stem cell with CD44hi/CD24lo phonotype is described having stem cell properties and represented as the main driving factor in breast cancer initiation, growth, metastasis and low response to anti-cancer agents. Glucoseregulated proteins (GRPs) are heat shock protein family chaperons that are charged with regulation of protein machinery and modulation of endoplasmic reticulum homeostasis whose important roles in stem cell development and invasion of various cancers have been demonstrated. Here, we investigated the expression levels of GRP78 and GRP94 in CD44hi/CD24lo phenotype breast cancer stem cells (BCSCs). Methods: MCF7, T-47D and MDA-MB-231 breast cancer cell lines were used. CD44hi/CD24lo phenotype cell population were analyzed and sorted by fluorescence-activated cell sorting (FACS). Transcriptional and translational expression of GRP78 and GRP94 were investigated by western blotting and quantitative real time PCR. Results: Results showed different proportion of CD44hi/CD24lo phenotype cell population in their original bulk cells. The ranking of the cell lines in terms of CD44hi/CD24lo phenotype cell population was as MCF7

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分子伴侣GRP78和GRP94在CD44hi/CD24lo乳腺癌干细胞中的过表达

CD44hi/CD24lo表型的乳腺癌干细胞被认为具有干细胞特性，是乳腺癌发生、生长、转移和对抗癌药物低反应的主要驱动因素。糖调蛋白(Glucoseregulated proteins, GRPs)是热休克蛋白家族的伴侣蛋白，负责调节蛋白质机制和调节内质网稳态，在干细胞发育和各种癌症的侵袭中起着重要作用。在这里，我们研究了GRP78和GRP94在CD44hi/CD24lo表型乳腺癌干细胞(BCSCs)中的表达水平。方法:采用MCF7、T-47D和MDA-MB-231乳腺癌细胞系。采用荧光活化细胞分选(FACS)对CD44hi/CD24lo表型细胞群进行分析和分类。采用western blotting和实时荧光定量PCR检测GRP78和GRP94的转录和翻译表达。结果:结果显示，CD44hi/CD24lo表型细胞群在原体细胞中所占比例不同。细胞系CD44hi/CD24lo表型细胞群排序为MCF7

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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