DEVELOPMENT OF CONDUCTOMETRIC ARGININE-SENSITIVE BIOSENSOR BASED ON ARGININE DEIMINASE

Abstract

An arginine-sensitive conductometric biosensor was developed. Two pairs of gold interdigitated electrodes deposited on the ceramic substrate were used as a conductometric transducer. Arginine deiminase was used to create a bioselective membrane. The enzyme was immobilized on the sensitive surface of one pair of electrodes of the conductometric transducer by covalent crosslinking with glutaraldehyde; a reference membrane based on bovine serum albumin was deposited on the second pair of electrodes. The optimal conditions of enzyme immobilization (enzyme concentration, duration and method of immobilization) were determined. The influence of solution parameters (ionic strength, buffer capacity, pH and protein concentration) on the operation of the developed biosensor was studied as well as stability of its functioning and storage. The iosensor  selectivity and main analytical characteristics detection limit, linear and dynamic ranges of the iosensor operation etc.) were investigated. The proposed biosensor was shown to be promising for the quantitative determination of arginine content in real samples.