COMPLEX EFFECT OF LOW-INTENSITY LASER RADIATION AND POTASSIUM CHANNEL PEPTIDE INHIBITOR ON MELANOMA CELL SURVIVAL

Abstract

Melanoma is characterized by an aggressive development and a large number of metastases during diagnostics. Photodynamic therapy (PDT) is used to treat this type of cancer. However, the accumulation of photosensitizers is observed not only in malignant tumors, but also in high metabolic rate organs. Shortcomings of melanoma therapy can be eliminated using the complex effect of laser radiation and local administration of inhibitors of cellular processes. The goal. To study the complex effect of low-intensity laser radiation and potassium channel peptide inhibitor on melanoma cell survival. Materials and Methods. A875 melanoma cells were exposed to Kappa-theraphotoxin-Gr1b toxin and laser irradiation. The authors examined the level of apoptosis and necrosis in cells using fluorescence microscopy techniques. The xCELLigence system was used to assess the cytotoxic response of A875 melanoma cells. Results. The maximum number of apoptotic and necrotic cells was observed in the group of patients with A875 tumor cells exposed to a combination of Kappa-TRTX-Gr1b toxin and laser radiation (wavelength=1265 nm). This is due to the inhibition of potassium channels of intracellular cell membranes by Kappa-TRTX-Gr1b peptide, which are associated with the apoptosis. Conclusion. Selective potassium channel inhibition under pathological processes can be regarded as a significant supplement to the superficial malignant neoplasm complex therapy. The combination of toxin and irradiation will make it possible to potentiate their action and avoid the main PDT disadvantages. This approach unites the benefits of the local administration and precise exposure on the malignant tumor.