BIOLOGICAL COMPATIBILITY AND PROLIFERATIVE POTENTIAL OF FIBROBLASTS SEEDED ON BIOCOMPOSITE FORMED USING LASER RADIATION AND SOLDERS

Abstract

The aim of the work was to determine the viability, proliferative potential and adhesive properties of fibroblasts seeded on the surface and in the volume of a biocomposite formed using laser radiation and solders in in vitro experiments. Materials and methods. For the study, Wistar rat fibroblasts obtained at the National Research Center of Epidemiology and Microbiology named after Honorary Academician N.F. Gamalei were used. The biocomposite was formed using laser radiation and solders. The viability of cells on the surface of the biocomposite was assessed using the multiparametric RTCA iCELLigence cell culture analysis system (USA). The cytotoxicity of the biocomposite was determined in combination with triphenyltetrazolium bromide (MTT test, Merck Sigma-Aldrich, Switzerland). The proliferative potential and adhesive properties of fibroblasts were studied using a FEI Helios NanoLab 650 scanning electron microscope. Results and conclusions. The viability of cells on the surface and in the volume of a biocomposite formed using laser radiation and solder was proved using an electrophysical system for analyzing cell cultures. The absence of cytotoxicity of the biocomposite under the action of triphenyltetrazolium bromide in the spectrophotometric MTT test was demonstrated. It was found that during incubation of rat fibroblasts in the volume of the biocomposite, cell death is not observed, but, on the contrary, their proliferative potential is stimulated by increasing adhesion, which contributes to the formation of a dense cell layer. Conclusions. Biocomposite as a whole, as well as its individual elements, creates a favorable environment for the growth of fibroblast culture and can be used to restore the integrity of blood vessels using laser radiation and solder.