EVALUASI SEDIAAN MASKER ANTIOKSIDAN DARI EKSTRAK DAUN TEH HIJAU (CAMELLIA SINENSIS)

Desi Nawangsari
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Abstract

Abstract Green tea (Camellia sinensis L.) has the main efficacy as an antioxidant and contains polyphenol compounds in green tea leaves with the main component of polyphenol fraction namely Epigallocatechin-3-gallate (EGCG) which has strong activity to prevent free radicals that cause premature aging. Research has been carried out on the formulation of maskantioxidant preparations containing green tea leaf extract. The study begins with testing the antioxidant activity of ethanol extract of green tea leaves with a free radical reduction method of 1,1-diphenyl-2-picrylhydrazyl using visible spectrophotometry. Testing of antioxidant activity showed that the ethanol extract of green tea leaves gave an IC50 value of 3.17µg / mL. Formulations of gel masks made were F0 (base without active substances), F1 (base + concentration of ethanol extract of green tea leaves for IC50 (0,000317%)), F2 (base + concentration of ethanol extract of green tea leaves for 50xIC50 (0, 0158%)), F3 (base + concentration of ethanol extract of green tea leaves for 100xIC50 (0.0317%)), F4 (base + concentration of ethanol extract of green tea leaves for IC50), F5 (base + concentration of ethanol extract of green tea leaves worth IC50), F6 (base + concentration of ethanol extract of green tea leaves for IC50. Evaluation of gel mask preparations include, organoleptic examination and homogeneity, pH, viscosity, dry time of preparation and testing of antioxidant stability. F2 provides better and higher free radical reduction activity than products on the market. F4, F5, F6 shows an increase in free radical reduction activity.   Keywords: Green tea leaves (Camellia sinensis L.), DPPh, IC50 gel mask.
剂包从绿茶提取物(山茱丽亚SINENSIS)中提取抗氧化面膜
绿茶(Camellia sinensis L.)具有抗氧化剂的主要功效,绿茶中含有多酚类化合物,多酚部分的主要成分是表没食子儿茶素-3-没食子酸酯(EGCG),它具有很强的抗自由基活性。对含有绿茶提取物的面膜抗氧化制剂的配方进行了研究。本研究首先采用可见光分光光度法,采用1,1-二苯基-2-苦酰肼自由基还原法检测绿茶乙醇提取物的抗氧化活性。抗氧化活性测试表明,绿茶乙醇提取物的IC50值为3.17µg / mL,制备的凝胶膜配方为F0(无活性物质的底料)、F1(底料+绿茶乙醇提取物浓度为IC50(0,000317%))、F2(底料+绿茶乙醇提取物浓度为50xIC50(0,0158%))、F3(底料+绿茶乙醇提取物浓度为100xIC50(0.0317%))、F4(碱+ IC50绿茶乙醇提取物浓度),F5(碱+ IC50绿茶乙醇提取物浓度),F6(碱+ IC50绿茶乙醇提取物浓度)。凝胶膜制剂的评价包括感官检查和均匀性、pH值、粘度、干燥时间和抗氧化稳定性测试。与市面上的产品相比,F2具有更好、更高的自由基还原活性。F4、F5、F6自由基还原活性增强。关键词:绿茶,DPPh, IC50凝胶面膜
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