Genetic diversity of Fusarium oxysporum f. sp. lini by inter simple sequence repeat (ISSR)

Abstract

The genetic diversity in Fusarium oxysporum f . sp. lini from three natural populations (from Hebei, Ningxia and Inner Mongolia) was investigated by inter simple sequence repeat(ISSR). A total of 48 samples from 3 provinces were amplified using selected 10 primers. The band amplified by each primer ranged from 21 to 44, with the molecular weight between 250 and 5,000 bp. In total 316 bands were observed, 314 were polymorphic (99.37%). Nei's gene diversity (H) ranged from 0.0029 to 0.1994, and Shannon's information index (I) ranged from 0.0478 to 0.1809. Genetic diversity was greater within Ningxia populations (H = 0.1205, I = 0.1861). Unweighted pair-group mean analysis (UPGMA) indicated that clustering of Fusarium oxysporum f . sp. lini individuals mainly relates to their populations and geographic distances separating those populations. Genetic differentiation was indicated by ISSR analysis.