Compensating the effects of light attenuation in confocal microscopy by histogram modelling techniques

2008 2nd ICTON Mediterranean Winter Pub Date : 2008-12-01 DOI:10.1109/ICTONMW.2008.4773118

S. Stanciu, J. Friedmann

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引用次数: 8

Abstract

Confocal Laser Scanning Microscopy (CLSM) enables us to capture images representing optical section on the volume of a specimen. The images captured from different layers have a different contrast: the images obtained from the deeper layers of the specimen will have a lower contrast and will present a certain degree of blur in respect to the images obtained from the topmost layers. The main reasons responsible for the effects described above are light absorption and scattering by the objects and particles that are present in the volume through which the light passes. Also light attenuation can be caused by the inclination of the observed surface. In the case of the surfaces that have a steep inclination the reflected light will have a different direction than the one of the detector. We propose a technique that can be used to compensate the effects of light attenuation based on histogram operations. We will consider two types of histogram operations: histogram equalization and histogram specification. A discussion of the results that were obtained using the two types of operations will be presented.

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用直方图建模技术补偿共聚焦显微镜中光衰减的影响

共聚焦激光扫描显微镜(CLSM)使我们能够捕获图像，代表一个标本的体积上的光学部分。从不同层捕获的图像具有不同的对比度:从样品的较深层获得的图像将具有较低的对比度，并且相对于从最顶层获得的图像将呈现一定程度的模糊。造成上述效应的主要原因是光通过的体积中存在的物体和粒子对光的吸收和散射。此外，被观测表面的倾斜也会引起光衰减。在表面有一个陡峭的倾斜的情况下，反射光将有一个不同的方向与一个探测器。我们提出了一种基于直方图运算来补偿光衰减影响的技术。我们将考虑两种直方图操作:直方图均衡化和直方图规范。本文将讨论使用这两种操作所得到的结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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2008 2nd ICTON Mediterranean Winter

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