COMPARATIVE SEED STORAGE PROFILING OF SELECTED SPECIES OF THE GENUS VIGNA

Abstract

Seed storage profiling of some selected species/varieties of the genus Vigna was performed by Native and SDS PAGE. Germplasm of its 10 varieties were biochemically analysed for seed soluble protein content and their electrophoretic characterization. In electrophoretic characterization of seed proteins, Native PAGE and SDS PAGE were performed, respectively. Native PAGE and SDS PAGE were analysed by preparing a zymogram. An UPGMA dendrogram showing the genetic relationships among selected Vigna germplasm obtained by using the SDS-PAGE pattern of seed storage proteins was prepared. The germplasm was characterized biochemically by analysing protein polymorphism profile through Native and SDS PAGE. The soluble seed protein content values ranged from 36.23 to 57.53 mg/g. The protein polymorphism revealed a wide variability with respect to the number and mobility of bands. The seed proteins of Vigna species studied on Native PAGE exhibited the existence of 42 protein bands located in three zones namely A, B and C. All the selected species of the Vigna evaluated by SDS-PAGE exhibited a considerable genetic variation and hence the results obtained could be of broader spectrum.