Quality Assessment of Platelet Concentrates Prepared after Whole Blood Overnight Storage

Abstract

The present study focuses to assess the in vitro effect on quality of platelet concentrates (PCs) prepared from overnight-held whole blood (WB). Method: Thirty four units of WB were stored overnight at 20 to 24oC and processed on the following day, and 30 units of WB were processed immediately. Result: The volume of PCs from overnight-held WB on Day 1 was significantly lower. On the same day, the PCs were significantly lower in platelet count but by Day 3 and Day 5, the difference was disappeared. PCs made from overnight-held WB had a significantly higher total red blood count on Day 1, Day 3 and Day 5, and for total white blood count was significantly higher on Day 5. pH was significantly lower on Day 1, Day 3 and Day 5. Sterility test for both groups of PCs give negative results. Conclusion: PRP-derived PCs from overnight-held WB have significantly different in vitro variables as from freshly processed WB. However, based on one-sample t-test comparison with the quality requirement of PCs, the use of the PCs from overnight-held WB was possible as the results of volume, platelet count and pH were significantly higher than the quality requirement and specification. 