Frequency of Isolation and Antibiotic Resistance Patterns of Bacterial Isolates from Wound Infections, National Cancer Institute, Wad-Medani, Sudan, 2021

Abstract

Background: Disturbance of skin integrity (wound) provides a suitable environment for the growth and multiplication of bacteria. The emerging occurrence and spread of multidrug-resistant microbial pathogens present a significant challenge in providing effective health care and is, therefore, a worldwide medical issue. Aim: The current study reports on the occurrence of possible pathogens associated with wound infections at National Cancer Institute, Wad Madani, Sudan. Methodology: This was a cross-sectional descriptive, hospital-based study including 100 patients with wounds suspected to be infected. Suspicion was dependent on the inability of wounds to heal properly. A structured questionnaire was given to the subjects to acquire demographic information (age, gender, marital status) and the history of antibiotics administration. Wound swabs were collected and processed according to standard procedure and isolated bacteria were tested for their antimicrobial susceptibilities through the disc diffusion technique according to the CLSI guidelines. Results: Morphological identification revealed that the percentage of gram-positive cocci among the isolates was: S. Aureus (66%), and Streptococci (34%). On the other hand, gram-negative bacilli included the following: E coli (48.1%), Proteus vulgaris (19%), Proteus mirabilis (14%), Klebsiella species (9%), Pseudomonas species (5%) and other bacterial species (4%). Most bacterial isolates exhibited a high rate of resistance to cephalosporin generations (Cefixime (69%), Cefuroxime (49%), Cefotaxime (32%), Ceftriaxone (29%), Cefepime (20%)). The majority of bacterial isolates were sensitive to Meropenem (resistance rate 3%). The antibiogram results of the other antibiotics were as follows: Ciprofloxacin (18%), Gentamicin (16%). Conclusion: S. Aureus was the most prevalent bacteria among the isolates with a percentage of 66%. The highest rate of resistance was observed among cephalosporin generations, mainly Cefixime and Cefuroxime with resistance rate of 69% and 49%, respectively.