Immunohistochemical evaluation of inducible nitric oxide synthase (iNOS) expression in the dental follicle, follicular cyst, and calcifying cystic odontogenic tumor

International Journal of Dental Science and Research Pub Date : 2016-01-01 DOI:10.1016/j.ijdsr.2015.11.007

Safoura Seifi , Tahere Padeganeh , Syed Razie Rezaee , Ali Bijani

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Abstract

Objective

The calcifying cystic odontogenic tumor (CCOT) is a benign cystic neoplasm with a variety of histopathological and clinical features in comparison with the follicular cyst. iNOS is an enzyme that produces free radicals and is a mediator regulator of the inflammatory response. It has been implicated in tumorigenesis as well. The aim of this study was to compare iNOS expression in the dental follicle and two odontogenic lesions with different aggressive behaviors.

Methods

In this cross-sectional study, 44 paraffin blocks were selected from the dental follicle, follicular cyst, and CCOT, and immunohistochemical staining was done by iNOS. The percentage and intensity of staining (total score) were calculated in the cytoplasm of the epithelial cells.

Results

The highest total score of iNOS staining was found in the cytoplasm of the epithelial cells of CCOT when compared to the follicular cyst and dental follicle (p = 0.000). There was a significant difference in the final score between the follicular cyst and dental follicle (p = 0.001).

Discussion

The overexpression of iNOS in the CCOTs plays a role in its pathogenesis and iNOS, because of producing free radicals and damaging the oral tissue, may contribute to more aggressive behaviors of CCOT.

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诱导型一氧化氮合酶(iNOS)在牙滤泡、滤泡囊肿和钙化囊性牙源性肿瘤中的表达的免疫组化评价

目的钙化囊性牙源性肿瘤(CCOT)是一种良性囊性肿瘤，与滤泡性囊肿相比具有多种组织病理学和临床特征。iNOS是一种产生自由基的酶，是炎症反应的调节剂。它也与肿瘤发生有关。本研究的目的是比较不同攻击行为的牙源性病变和牙毛囊中iNOS的表达。方法在横断面研究中，从牙毛囊、牙毛囊囊肿和牙cot中选取44个石蜡块，用iNOS进行免疫组织化学染色。计算上皮细胞细胞质中染色百分率和染色强度(总分)。结果CCOT上皮细胞胞浆中iNOS染色总分高于滤泡囊肿和牙滤泡(p = 0.000)。滤泡囊肿与牙滤泡的最终评分差异有统计学意义(p = 0.001)。在CCOT中iNOS的过度表达在其发病机制中起作用，iNOS由于产生自由基和损伤口腔组织，可能导致CCOT更具攻击性的行为。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Dental Science and Research

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