THE EFFECT OF OVARIECTOMY ON THYROID C CELLS OF ADULT RATS

Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry Pub Date : 1900-01-01 DOI:10.2298/JMH0204345F

B. Filipović, B. Šošić-Jurjević, M. Manojlović-Stojanoski, N. Nestorović, V. Milošević, M. Sekulić

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引用次数: 5

Abstract

Summary: The structure and function of C cells of adult female rats after ovariectomy (Ovx) were investigated. Intact control and Ovx rats were i.p. treated with sterile olive oil for 4 weeks. Peroxidase-antiperoxidase (PAP) immunohistochemical procedure was applied to localize calcitonin (CT) in thyroid C cells while its serum content was determined by RIA method. Morphometric analyses of the C cells volume, that of their nuclei and relative volume density included stereological method with the multipurpose test system M42. Also, the average number of C cells number per mm2 was calculated. Ovx led to a significant increase in body weight (21%; p<0.005). At the same time the C cells of Ovx rats had a significantly decreased cell volume (13%; p<0.005) and their number per mm2 was increased by 59% (p<0.001) in comparison with the controls. Ovx resulted in reduction of serum CT level by 45% comparing to the corresponding controls. Based on these data it can be concluded that Ovx inhibits both the structure and function of the C cells.

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卵巢切除对成年大鼠甲状腺c细胞的影响

摘要:本文研究了卵巢切除后成年雌性大鼠C细胞的结构和功能。对照组和Ovx大鼠用无菌橄榄油灌胃4周。采用过氧化物酶-抗过氧化物酶(PAP)免疫组化方法定位甲状腺C细胞降钙素(CT)， RIA法测定血清降钙素含量。用M42多功能测试系统对C细胞体积、细胞核和相对体积密度进行立体学分析。并计算每mm2中C细胞的平均数量。Ovx导致体重显著增加(21%;p < 0.005)。同时Ovx大鼠的C细胞体积明显减少(13%;P <0.005)，每mm2的数量比对照组增加了59% (P <0.001)。与相应的对照组相比，Ovx导致血清CT水平降低45%。根据这些数据可以得出结论，Ovx抑制C细胞的结构和功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry

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