The Comparison of Routine Hematology Test Between Veins and Capillary Blood: Studies on Hematology Analyzer

Abstract

Routine hematological parameters is a basic assessment of blood component, which determines the amount, variation, percentage, concentration, and quality of blood. The type of sample used was venous blood with EDTA anticoagulants. Currently there is a hematology analyzer which analyses capillary blood for routine hematological parameters. This research aims to determine the feasibility of capillary blood samples as an alternative sample on routine hematological parameters using Medonic M-series M32. The method used in this research was comparative analytic cross sectional. Venous and capillary blood from 70 healthy students of D-IV MLT Health Polytechnic of Jakarta III was used in this research. The researchers took 3 mL of venous blood and 20 μL of capillary blood. Then, the researchers performed the routine hematological parameters on the hematology analyzer. The results were calculated using independent t test (normal data distribution) or Mann-Whitney test (abnormal data distribution). The hypothesis used was 90% of interval confidence (α = 0.1). The results of significant difference test shows the probability value (p) on parameters Hb, Ht, leukocyte and erythrocytes count were more than α (0.1). Thus, there is no significant difference between venous and capillary blood for the parameters. The p value for platelet count is less than α (0.1), and as the results, it shows that there is significant difference between venous and capillary blood samples. The conclusions of this research are capillary blood can be used as an alternative sample for the parameters hemoglobin, hematocrit, leukocyte and erythrocytes count using hematology analyzer Medonic M-series M32. On the contrary, capillary blood is not recommended for platelet count. Keyword: hematology analyzer, capillary blood, venous blood