Lei Xing, Cong-Lin Zhao, Han-Zhang Mou, JianBin Pan, Bin Kang*, Hong-Yuan Chen and Jing-Juan Xu*,
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引用次数: 0
Abstract
Developing an imaging method with micrometer-to-subcellular resolution is of great significance for visualizing biological samples of different sizes. The label-free and high-throughput mass spectrometry imaging (MSI) technology has shown potential in the implementation of this view. Despite many improvements in MSI witnessed over the past decades, it remains a challenge to achieve a flexible resolution from micrometer down to subcellular level with high detection sensitivity. In this Perspective, we focus on the recent development of MSI techniques based on different ionization resources. Furthermore, several designs of instruments and applications in bioimaging have been reviewed and compared. Additionally, we proposed the perspectives and challenges for MSI methods, including pursuing the matrix free and multiscale resolution with high detection sensitivity and deeply combining machine learning in omics research.
期刊介绍:
Chemical & Biomedical Imaging is a peer-reviewed open access journal devoted to the publication of cutting-edge research papers on all aspects of chemical and biomedical imaging. This interdisciplinary field sits at the intersection of chemistry physics biology materials engineering and medicine. The journal aims to bring together researchers from across these disciplines to address cutting-edge challenges of fundamental research and applications.Topics of particular interest include but are not limited to:Imaging of processes and reactionsImaging of nanoscale microscale and mesoscale materialsImaging of biological interactions and interfacesSingle-molecule and cellular imagingWhole-organ and whole-body imagingMolecular imaging probes and contrast agentsBioluminescence chemiluminescence and electrochemiluminescence imagingNanophotonics and imagingChemical tools for new imaging modalitiesChemical and imaging techniques in diagnosis and therapyImaging-guided drug deliveryAI and machine learning assisted imaging