Rhizosphere Streptomycetes Producing Antifungal to Candida albicans ATCC 10231 and C. albicans ATCC 24433 from Various Plants

Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia Pub Date : 1900-01-01 DOI:10.4108/EAI.2-5-2019.2284679

E. Sumiati, D. Anggraeni

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Abstract

A research on potency of rhizosphere streptomycetes producing antifungal on various plants (Cyperus rotundus L., Zea mays L., and Rhizopora mucronanata) was conducted. Twelve isolates of streptomycetes was tested to assess their potency in producing antifungal compound by using agar block method with Candida albicans ATCC 10231 and C. albicans ATCC 24433 as test fungi. The result showed that 3 isolates among 12 isolates showed their potency to produce antifungal. Based on their antifungal activity, 1 isolates (J10) were chosen to produce antifungal compound using fermentation method in GYE broth. Characterization of antifungal compound was carried out by using TLC, GC-MS and bioautographic test. The result of bioautography test showed that the spot from TLC of J10 isolates could inhibit C. albicans ATCC 10231 growth. Identification of antifungal compound of J10 by using TLC analysis and spray reagent suggested that then alkaloid, flavonoid, and phenol class of compound.

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产抗各种植物白色念珠菌ATCC 10231和白色念珠菌ATCC 24433的根际链菌

研究了根际链霉菌对不同植物(香柏、玉米和毛霉)的抑菌效力。以白色念珠菌ATCC 10231和白色念珠菌ATCC 24433为试菌，采用琼脂块法对12株链霉菌进行抑菌活性测定。结果表明，12株菌株中有3株具有抑菌活性。根据菌株J10的抑菌活性，选择1株菌株在GYE肉汤中发酵制备抑菌化合物。采用薄层色谱(TLC)、气相色谱-质谱(GC-MS)和生物自显影(bioautoography)等方法对抗菌化合物进行表征。生物图谱检测结果表明，J10分离株TLC斑点能抑制白色念珠菌ATCC 10231的生长。通过TLC分析和喷雾试剂对J10的抗真菌化合物进行鉴定，确定其为生物碱类、类黄酮类和酚类化合物。

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Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia

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