Comparison of the activity of boiling water extract of purple cabbage leaves and albendazole drug against protoscolices of Echinococcus granulosus In vitro study.

Abstract

Hydatid cystic disease is a financial burden in Iraq because it reduces the productivity of sheep, goats, cows, and camels by leaving their afflicted organs unsuitable for human consumption, causing weight loss and poor health. This study aimed to evaluate the efficacy and applicability of Brassica oleracea boiling water extract as an in-vitro anti-protoscolices therapy model to albendazole. Fresh leaves of purple cabbages (Brassica oleracea var. capitata) were extracted comprehensively by maceration in boiling water, and the required 100, 200, and 300 mg/ml concentrations were tested. Hydatid (human or animal) samples were collected from the hospital and external laboratories in Babylon province and stored in Kreb ringers culture media and cyst fluid (4:1) until use. For comparison, several concentrations of albendazole were generated. In three replications, the control group received one milliliter of distilled water and all of the extract and drug concentrations stated above. The viability rates of the protoscolices were determined for (0, 24, 48, 72, 96, 120, 144, 168, 192, 216) hours. According to the findings, the proportion of viable protoscolices was 84 % at zero time and 0 % on the ninth day after the experiment began. The boiling water extract for purple cabbages with a concentration of 300 mg/ml was shown to be the most successful in removing protoscolices viability at a percentage of 0.67% after 96 hours, compared to the albendazole medicine at a percentage of 0% after 120 hours. The findings of a boiling water extract from Brassica oleracea var. capitata revealed that protoscolices' viability had been effective. It can also be used as an alternative to chemotherapy in treating cyst hydatid infection. Keywords: Purple cabbages, Protoscolices, Echinococcosis, Albendazole, plant extract