Bacteriology Test to Detect Coliform Bacteria in Beverages Sold at University Cafeteria in Medan

Buletin Farmatera Pub Date : 2020-10-20 DOI:10.30596/BF.V5I3.3437.G4647

A. Simaremare

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引用次数: 1

Abstract

T The World Health Organization standard for clean and safe water is that 95% of the water samples examined do not contain coliform organisms per 100 mL. Contaminated beverages will lead to digestive tract diseases. Food poisoning cases come from contaminated can be sourced from school canteens, street vendors factories, households, family parties, and even hotels. One of the most frequent and sensitive laboratory tests to detect microorganisms contamination in water is a bacteriological examination. This research aimed to determine the microbiological quality of beverages sold in the university canteens in Medan. The microbiological examination is carried out by the Most Probable Number (MPN) method that consists of two stages, presumptive and confirmation test. From 15 samples of beverages tested, the percentage of samples contaminated with coliform bacteria was 100%, based on WHO criteria were low-risk category by 1 sample (6.7%), intermediate-risk by 5 samples (33.3%), and high-risk category by 9 samples (60%). Therefore, beverages sold in the canteen did not meet the condition for appropriateness to be consumed because of contamination by coliform bacteria.

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棉兰大学食堂售饮料中大肠菌群的细菌学检测

世界卫生组织对清洁和安全用水的标准是每100毫升水样中95%不含大肠菌群。受污染的饮料会导致消化道疾病。受污染的食物中毒病例可能来自学校食堂、街头摊贩工厂、家庭、家庭聚会，甚至酒店。细菌学检查是检测水中微生物污染最常见和最灵敏的实验室检测方法之一。本研究旨在确定棉兰大学食堂销售的饮料的微生物质量。微生物学检查采用最可能数(MPN)法进行，该方法包括两个阶段，假设试验和确认试验。在所检测的15份饮料样品中，大肠菌群污染的样品百分比为100%，根据世卫组织的标准，低风险类样品1份(6.7%)，中等风险类样品5份(33.3%)，高风险类样品9份(60%)。因此，食堂出售的饮料因受到大肠菌群污染而不符合适宜饮用的条件。

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Buletin Farmatera

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