DEVELOPMENT AND OPTIMIZATION OF CONSTRUCTION OF AMPEROMETRIC BIOSENSOR BASED ON THE GLUTAMATE OXIDASE FOR DETECTION OF GLUTAMATE IN THE SERUM SAMPLES

Abstract

In this study, an amperometric glutamate-sensitive biosensor has been optimized. The platinum disk electrode was used as a transduser. The enzyme glutamate oxidase that is covalent crosslinked with bovine serum albumin by glutaraldehyde was used as a bioselective element. Conditions of enzyme immobilization on the transducer surface were optimized (enzyme and glutaraldehyde concentrations and immobilization time). To test the possibility of this biosensos functioning in complex biological samples, the influence of the working solution parameters on the biosensor operation was investigated (concentration of the working buffer solutions, it`s temperature, presence of the protein in the analyzed sample). In addition, the selectivity of the developed biosensor with regarding to possible interference was tested. Linear range of biosensor was obtained in the range of 5 to 600 μM of glutamate and the sensitivity was 150-200 nA/mM. The minimum limit of detection was 2 μM glutamate. The obtained data indicate the possibility of application of developed biosensor for determination of content of glutamate in the serum samples.