Invitro Antihelmintic Activity of Individual and Combined Leaf Extracts (Punica granatum and Psidium guajava) Against Pheritima Posthuma

Abstract

Punica granatum and Psidium guajava leaves were easily available and commonly used in our day –to-day life. The present study aimed at the in-vitro comparative study of anthelmintic activity of aqueous extracts of leaves of Punica granatum, Psidium guajava and mixed. The fruits were extracted separately with distilled water by maceration method .The various concentrations of the extract (50,100, 200mg/ml) respectively were screened for their anthelmintic activity using Pheritima posthuma. The activity was comparable with the standard drug albendazole. When the concentrations of the extract are increased, a gradual increase in anthelmintic activity is observed. The study involved the determination of time of paralysis (P) and time of death (D) of the worms. Aqueous extract of Punica granatum, Psidium guajava and mixed showed anthelmintic activity but mixed extract was more efficient anthelmintic activity against Indian earthworms. The data were found statistically significant by using one way ANOVA (P< 0.001).