Carbon Fibers in Biomedical Applications

Abstract

Three-dimensional growth of fibroblasts on carbon fibre mesh and assessment of biocom - patibilty by in vitro and in vivo examination was done. Suitable size carbon fiber mesh after sterilization, placed in six well cell culture plate. The mesh was co-cultured with p-MEF cells. At different time intervals the viability and proliferation of the p-MEF cells was evaluated. The primary objective of this study was biological evaluation of carbon fibre mesh which can be used for creation of three-dimensional scaffolds for tissue engi -neering. Among the possible forms of implants, fibrous matrices are highly promising for the tissue regeneration by acting as a cell-supporting scaffold. Results of in vitro observa tions of the morphology p-MEF cells seeded on the surface of carbon fibre mesh shows adhesions and attachment of fibroblasts cells to carbon fibres on day 3 post seeding. They attached firmly and were uniformly spread along the fibres on day 5 postseeding and mostly spindle-shaped and cover almost all their surface on day 7 postseeding and such a spreading of cells indicates good adhesions and biocompatibility of carbon fibres. In vivo examination of retrieved sample on day 30 post implantation shows that carbon fibre mesh was covered by dense thick fibrous connective tissue. use of different types of carbon fibers of different physical, structural and chemical properties, resulting from many technological parameters. Most of the papers concerning examinations of CFs for medical purposes describe neither the type of carbon fibers used nor their funda mental properties determining their behavior in a biological environment. We will discuss the use of carbon fibers in biomedical applications under different headings: