Clinical Conditions and Risk Factors of Acinetobacter Baumannii Producing Metallo Beta-Lactamases Among Hospitalized Patients

Abstract

Purpose: The study aimed to determine the clinical conditions and risk factors associated with MBL produced by A.bumannii among hospitalized patients. Subjects and Methods: The clinical samples were collected from inpatients and subcultured on routine culture media for growth. Identification of bacteria along with antimicrobial sensitivity testing was done by VITEK®2 compact (bioMerieux). Isolates that were resistant to Meropenem and/or Imipenem were followed to detection of MBL by using metallo-β-lactamases by imipenem EDTA combined disc test (IMP-EDTA CDT) method. Demographic and clinical data of each patient were collected in terms of the type of infection, hospital-stay, associated factors, and outcome till discharge. Results: A number of 73(86.9%) isolates of A.baumannii were resistant to carbapenem. out of 73 carbapenem-resistant isolates, 64(87.7%) were found to be MBL positive. The patients with age more than 60 years i.e.  35.9% (23/64) were found to be more common in MBL positive isolates of A. baumannii. The difference in the distribution of MBL positive and MBL negative cases with endotracheal intubation and in Surgery during the last 30 days of incubation was found to be statistically significant. The mortality rate of patients infected by MBL positive isolates of A.baumannii was 12.5%. Conclusion: The MBL positive strains among carbapenem-resistant isolates of A.baumannii were high. endotracheal intubation and Surgery during the last 30 days were independently associated with MBL positive cases.