Avaliação da atividade antimicrobiana de óleo essencial de Cravo-da-Índia (Syzigium aromaticum) obtido com CO2 em fase supercrítica

Abstract

/ / / / / / método disco-difusão (CLSI, Todos os óleos essenciais demonstraram ter atividade antibacteriana, sendo o obtido 50°C / maior halo para os microrganismos Staphylococcus aureus, Escherichia coli ABSTRACT – In recent decades, humanity has been seeking healthier methods of life, especially in the food area, the search for species of plants rich in bioactive compounds have been encouraging the development of new researches. This study aimed to obtain clove India extracts ( S. aromaticum ) using CO 2 in supercritical phase and ascertain the in vitro antimicrobial activity. The clove extracts were obtained at operating temperature and pressure of 40 °C / 100bar, 40 °C / 200bar, 40 °C / 300bar 50 °C / 100bar, 50 °C / 200bar and 50 °C / 300bar. The main compound identified was Eugenol. Antimicrobial activity was assessed by disk diffusion method on solid medium (CLSI, 2004). All extracts have demonstrated antibacterial activity, and the extract obtained at 50 °C / 300bar presented the largest inhibition zone for Staphylococcus aureus , Escherichia coli and Candida albicans .