DETECTION OF CLOBAZAM AND ITS METABOLITE IN URINE DURING POISONING USING HPLC-QqQ-MS/MS

Abstract

Background. The problem of determining the fact of acute and lethal poisoning with clobazam is still an urgent task of analytical toxicology. The medicinal substance clobazam belongs to the group of benzodiazepines, which is included in the list of psychoactive substances whose turnover is limited, since it has a high toxicity profile in overdose and abuse. Aims. To propose a simple, reliable and sensitive technique for the identification of clobazam and its metabolite in urine by the modern HPLC-QqQ-MS/MS method. Materials and methods. We have described a simple and sensitive HPLC-QqQ-MS/MS method for qualitative determination of clobazam in urine. Results. According to the results of the study, the retention time in the selected chromatography conditions for clobazam was 5.17 minutes, and its metabolite (norclobazam) found in urine was 4.56 minutes. The base peak for clobazam is 259 m/z, and for norclobazam it is 245 m/z. Conclusion. For the first time, a study is presented, which provides a validated method of chemical-toxicological examination for poisoning with clobazam by HPLC-MS /MS, tested both on a model mixture and on a real biological matrix of the patient's urine after taking clobazam. This technique can be used as a confirmatory research method and is an addition to the clinical picture in forensic medical examination. Keywords: clobazam, HPLC-QqQ-MS / MS, detection, urine sample preparation