Secretome And Ramiprilat Effects On Endothelial Progenitor Cells Proliferation In Chronic Coronary Syndrome Patient

Abstract

It is known, that patients with chronic coronary syndrome (CCS) have lower endothelial progenitor cells (EPC) levels compared with healthy people. Secretome is a paracrine product from human umbilical cord blood mesenchymal stem cells (hUCB-MSC) that contains growth factors that enhanced the neovascularization and angiogenesis. Ramiprilat is an active form of ramipril which acts as an angiotensisn-converting enzyme inhibitor (ACE-I). Ramipril has been shown to have an effect on EPC proliferation. The effect of secretome alone or combination with ramiprilat is not yet elucidated. This study aimed to identify the effect of secretome compared with ramiprilat on EPC proliferation. The EPC was collected from a CCS person and cultered in stemline II medium for seven days. Cultured EPC were then divided into 8 groups of control, various doses of secretome (2, 10, and 20%), ramiprilat 10 μmol, and combination of various doses of secretome and ramiprilat. Three days after the treatement, the EPC proliferation measured by MTT assay in 96-well plated. The result of this study shown the higher the dose of secretome (2,10, and 20%) increase the proliferation of EPC better than ramiprilat (0.7128; 1.2417; 1.585 OD vs 0.7 OD; p<0.001). in conclusion secretome works in dose-dependent manner, the higher the concentration of secretome, the higher the EPC proliferation.