Spectroscopic Characterization of Biological Tissue using Quantitative Acoustics Technique

Abstract

This paper presents a new technique for imaging spectroscopic characterization of different biological tissues using Quantitative Acoustics Technique (QAT). This colorimetric study is imposed on different biological tissues like onion, human skin etc. where images are taken by a digital camera. The method is based on the hypothesis that the color spectrums and relative intensities differ for directly transmitted light and light scattered at an angle which give the tissue characteristics. In order to attain that an indigenous device is designed to pass a collimated beam of light from a white LED through a slide containing the tissue. The values for intensities of the primary colors Red, Green and Blue in the region of the tissues are extracted from the image for both positions. An analysis of these color and intensity values provides information on the morphological structure of the tissue and their optical characteristics. For preliminary testing of the system, some non-biological material (like filtered paper) is used. Scattering prospect of red is higher in color and rubbed filter while in jelly blue is scattered most. This result is compatible with the theoretical perspective.