Uji Presisi Kadar FE Pada Asi Metode Spektrofotometri Serapan Atom

Nazula Rahma Shafriani
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Abstract

One of the nutrients that babies need is iron (Fe). Iron content can be measured by atomic absorption spectrophotometry (AAS). This method is often used because it is simpler, more accurate and has high precision. This study was to determine iron levels and to determine precision of examination of iron levels in breast milk using AAS. The method of this research is the preparation of samples, tools, and materials, the manufacture of a standard solution of iron (Fe), and the calculation of the precision value of the examination of iron levels in breast milk. According to the findings, the three test groups' average Fe level was 1.1486 mg/L. This study's precision test was run based on repeatability. In this study, the precision test was conducted by measuring three groups of samples ten times. The precision test based on repeatability is declared acceptable by comparing the CV value obtained with CV-Horwitz, if the %CV obtained is less than CV-Horwitz, then the precision test based on repeatability is acceptable. Based on the measurements that have been made, CV (%) in test group A is 7.0690, test group B is 9.0686, test group C is 7.9807. These results indicate that the three test groups have CV % which is smaller than CV-Horwitz. Depending on the findings of the study, the validation of the technique for calculating Fe levels using AAS satisfies the standards for the approved value.
检测原子吸收光谱测定法法上的FE精度
婴儿需要的营养素之一是铁(Fe)。铁的含量可用原子吸收分光光度法测定。这种方法简单、准确、精密度高,因此常被采用。本研究旨在测定母乳中铁含量,并确定原子吸收光谱法测定母乳中铁含量的准确性。本研究的方法是准备样品、工具和材料,制作铁(Fe)标准溶液,计算母乳中铁含量检测的精度值。结果表明,3个试验组的平均铁含量为1.1486 mg/L。本研究的精度测试是基于可重复性进行的。在本研究中,通过对三组样品进行10次测量来进行精度测试。通过将所得CV值与CV- horwitz进行比较,表明基于重复性的精密度试验是可以接受的,如果所得CV %小于CV- horwitz,则基于重复性的精密度试验是可以接受的。根据已做的测量,A组的CV(%)为7.0690,B组为9.0686,C组为7.9807。这些结果表明,三个试验组的CV %都小于CV- horwitz。根据研究结果,使用原子吸收法计算铁水平的技术验证符合批准值的标准。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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