THE EFFECTIVENESS OF SOURSOP/GRAVIOLA LEAF EXTRACT (ANNONA MURICATA L.) ON THE LEVEL OF RAT PANCREATIC MALONDIALDEHYDE (RATTUS NORVEGICUS) DIABETIC HYPERCHOLESTEROLEMIA INDUCTED BY ALLOXAN

Abstract

Background: Hyperglycemia in diabetes mellitus causes oxidative stress which increases free radical production. The presence of higher malondialdehyde (MDA) level shows the increase of free radicals. Soursop leaf can act as an antidiabetic agent based on its role as antioxidant that binds free radicals and decreases MDA level. The aim of this research was to prove the effectiveness of soursop leaf extract (Annona muricata L.) on pancreatic malondialdehyde (MDA) level in alloxan-induced diabetic rats (Rattus norvegicus). Subjects and Method: The subjects in this research were 30 rats and divided into five groups of treatment ie group I (Negative Control/KI), which was given distilled water and standard feed, group II (Positive Control/KII), which was given vitamin E 150 IU/kgBW/day, group III (KIII), which was given soursop leaf extract 75 mg/kgBW/day, group IV (KIV), which was given soursop leaf extract 150 mg/kgBW/day, and group V (KV), which was given soursop leaf extract 300 mg/kgBW/day. Each group consisted of 5 rats and was given soursop leaf extract for 21 days after being induced by alloxan and high-fat diet. Kruskal-Wallis test and Mann-Whitney test were used to process the data. Results: Mann-whitney test showed significant difference in KI compared to KII, KIII, KIV, and KV due to their significance values being <0.05 (p <0.05). Conclusion: soursop leaf extract 150 mg/kgBW/day is the most effective dose on pancreatic MDA levelreduction.