Activity of Water Soluble Polysaccharide White Bentul (Colocasia Esculenta Schott [L]) As a Candidate for Antidiabetic Agent

Abstract

White bentul tuber is one of tuber plant species which have bioactive compound of Water Soluble Polysaccharide (WSP) and potentially healthy nutrition in the therapy of metabolic syndrome disease. The purpose of this research is to prove the ability of WSP isolate to reduce blood glucose level in white mice.  Research method include the yield of WSP isolates white bentul tuber using enzymatic method, WSP identification using HPLC with Aminex HPX-87C BIORAD5 columns, and antidiabetic activity test using white mices. Test activity was performed in six treatment groups (Normal, Induction STZ 20 mg/ kgBW, Induction STZ 20 mg / kgBW + metformin 195mg / KgBW, three treatment with STZ induction 20 mg/ kgBW and WSP isolate with concentration 200, 400, and 600mg / kgBW). Determination of blood glucose levels using glucometer and supported by observation of histologic improvement of beta pancreatic cells in white mice that have necrosis. The result research are WSP yield of 4.81% and WSP level of 94.45%. Results of blood glucose levels of mice induced STZ 20mg/kgBW decreased optimal blood glucose with a dose of WSP 400mg/kgBW in the first week and histologic improvement of beta pancreatic cells that experienced the most optimal necrosis at WSP dose of 200 mg/kgBW. The conclusion of this research is the provision WSP isolate of white bentul tubers at doses of 200, 400, 600 mg/kgBW can decrease the blood glucose level induced STZ 20mg/kg BW and histological improvement in pancreatic beta cells at the most optimal dose of 200 mg / kgKeywords: White bentul tuber, water soluble polysaccharides, diabetes mellitus, beta pancratic cells