Interaction between living neuronal network and outer world by programmable multisite stimulation system

S. Kudoh, A. Kiyohara, C. Hosokawa, T. Taguchi, I. Hayashi
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引用次数: 0

Abstract

Rat hippocampal neurons organized complex networks on a culture dish which has 64 planner microelectrodes and action potentials of neurons were recorded from an individual electrode. MED64 system, one of multi site recording systems for extracellular action potential was used for recording network activities of living neuronal networks and for applying inputs from outer world to the network. Neurons reconstructed complicated networks along culture days, and a spatio-temporal pattern of spontaneous action potentials was drastically changed after transient high-frequency bursting activities (HFB). After HFB, spatial distribution of spontaneous action potentials changed to heterogeneous one. We designed programmable multisite stimulation head amplifier, and analysis of evoked action potentials induced by the stimulator confirmed that there are some hub-like structure which has many inputs from other neurons. In addition we are developing "biomodeling system", in which living neuronal network connected to moving robot.
活体神经元网络与外部世界的可编程多点刺激交互作用
大鼠海马神经元在64个规划微电极培养皿上组织了复杂的网络,并从单个电极上记录神经元的动作电位。MED64系统是细胞外动作电位多位点记录系统之一,用于记录活体神经元网络的网络活动,并将外界输入应用于网络。随着培养时间的延长,神经元重构了复杂的神经网络,瞬时高频爆发活动(HFB)后自发性动作电位的时空格局发生了显著变化。HFB后,自发性动作电位的空间分布转变为异质分布。我们设计了可编程的多点刺激头放大器,通过对刺激器诱发的动作电位的分析,证实了脑内存在一些中枢状结构,该结构可以接收来自其他神经元的多个输入。此外,我们正在开发“生物建模系统”,其中活的神经网络连接到移动的机器人。
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