Prevalence of Staphylococcus aureus contamination in bovine milk from local dairy herd in Oyo State, Nigeria

Tropical veterinarian Pub Date : 2018-08-21 DOI:10.4314/TV.V36I1

I. O. Olatoye, U. Uba, S. Akintunde

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Abstract

Unhygienic practices, handling and transportation; pooling of milk from unscreened mastitic cows and indiscriminate prophylactic, therapeutic and growth promotion use of antibiotics in Nigerian cattle production could result in dissemination of resistant bacteria including Staphylococcus aureus and leading to food poisoning. This work aims to determine the prevalence of Methicllin Resistant Staphylococcus aureus (MRSA) in bovine milk from local herds. A total of 165 fresh bulk milk samples were collected from local herds at Ibarapa, Oyo and Oke-Ogun in Oyo State for isolation of Staphylococcus aureus. Standard bacteriological methods were used to determine the bacterial load, isolate and characterize Staphylococcus aureus in fresh bovine bulk milk from dairy herds in Oyo State. Antibiotics sensitivity of the isolates was determined using disc diffusion method. Bacteriological assay revealed high total mean bacterial counts ranging from 1.5+0.069 to 6.1+23.19 log cfu/ml, with the highest contamination in 13.9% of milk from Oke-Ogun, followed by 4.2% from Oyo and Ibarapa respectively. There was a prevalence of 31.5% coagulase positive Staphylococcus aureus with 7.9% isolates indentified as MSRA. The isolates showed very high resistance to several antibiotics with the highest resistance to augmentin (88.5%) and the highest sensitivity to ofloxacin (96.1%). This study established the presence of Staphylococcus aureus in milk from local herds with a prevalence of 33.1%. This signified the high risk of mastitis in the dairy cows from the study area. The high prevalence of MRSA is of food safety concern and could have resulted from indiscriminate use of antibiotics. Enforcement of milk hygiene practices, constant screening and treatment of mastitic cow as well as effective milk pasteurization are hereby suggested to ensure food safety.Keywords: Animal, Antimicrobial agents, Bulk milk, Staphylococcus aureus

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尼日利亚奥约州当地奶牛群的牛奶中金黄色葡萄球菌污染的流行情况

不卫生的操作、处理和运输;在尼日利亚的养牛生产中，将未经筛选的乳腺炎奶牛的乳汁汇集在一起，以及不加区分地在预防、治疗和促进生长方面使用抗生素，可能导致包括金黄色葡萄球菌在内的耐药细菌的传播，并导致食物中毒。这项工作的目的是确定流行的耐甲氧西林金黄色葡萄球菌(MRSA)从当地牛群的牛奶。从Oyo州Ibarapa、Oyo和Oke-Ogun的当地牛群中采集了165份新鲜散装牛奶样本，用于分离金黄色葡萄球菌。采用标准细菌学方法确定Oyo州奶牛群新鲜牛乳中的细菌载量，分离并表征金黄色葡萄球菌。采用圆盘扩散法测定菌株对抗生素的敏感性。细菌学检测结果显示，总平均细菌计数在1.5+0.069 ~ 6.1+23.19 log cfu/ml之间，其中Oke-Ogun的污染最高，为13.9%，其次是Oyo和Ibarapa，分别为4.2%。凝固酶阳性金黄色葡萄球菌患病率为31.5%，其中7.9%的分离株被鉴定为MSRA。该菌株对多种抗生素均表现出很高的耐药性，其中对augmentin的耐药性最高(88.5%)，对氧氟沙星的敏感性最高(96.1%)。本研究确定了当地牛群的牛奶中存在金黄色葡萄球菌，患病率为33.1%。这表明研究区域的奶牛患乳腺炎的风险很高。耐甲氧西林金黄色葡萄球菌的高流行率是食品安全问题，可能是由于滥用抗生素造成的。建议加强牛奶卫生规范，不断筛查和治疗乳牛，以及有效的牛奶巴氏消毒，以确保食品安全。关键词:动物，抗菌药物，散装奶，金黄色葡萄球菌

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Tropical veterinarian

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