Differentiation and Monitoring of Cells Using a Biochip for Regenerative Medicine

T. Uchida, F. Arai, O. Suzuki, A. Ichikawa, T. Fukuda, T. Katagiri, R. Kamijo, Masanori Nakamura, M. Numata, N. Watanabe
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引用次数: 5

Abstract

A novel biochip is developed for culturing stem cells. Biochip is made of Polymer (PDMS), and cells can be loaded by gradient strains in one chip. They grow well on a hydrophilic membrane and differentiation is promoted by cyclic strains. In this paper, we propose the method for culturing and monitoring of stem cells such as bone marrow stromal cells (ST2 cells) and myoblasts (C2C12 cells), and the results of culture. First we analyzed strains on a membrane when an air hole is decompressed, and clarified their range. From experiment, bone marrow stromal cells grew well in a narrow range, and we quantified their ALP activity as a measure of differentiation. As myoblasts, the direction of their differentiation was perpendicular to a groove, that is, the same direction of uniaxial strains.
再生医学用生物芯片的细胞分化与监测
研制了一种用于培养干细胞的新型生物芯片。生物芯片是由聚合物(PDMS)制成的,细胞可以通过梯度应变加载在一个芯片上。它们在亲水性膜上生长良好,循环菌株促进分化。本文提出了骨髓基质细胞(ST2细胞)和成肌细胞(C2C12细胞)等干细胞的培养和监测方法,以及培养结果。首先,我们分析了空气孔减压时膜上的应变,并澄清了它们的范围。从实验中,骨髓基质细胞在狭窄的范围内生长良好,我们量化了它们的ALP活性作为分化的衡量标准。作为成肌细胞,它们的分化方向垂直于一个凹槽,即与单轴应变方向相同。
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