The Effect of Bacillus Coagulans as Feed Probiotics on Non-specific Immunity of Whiteleg Shrimp Litopenaeus Vannamei

Abstract

The study aimed to evaluate the effect of giving a different dosage of Bacillus coagulans to feed (feed probiotics) on nonspecific immunity of Litopenaeus vannamei (white shrimp). The parameters tested included water quality and shrimp immunity parameters, namely total hemocyte count (THC), differential hemocyte count (DHC) and phagocytosis activity (PA) and carried out for 20 days of culture. The method of administering Bacillus coagulans was by spraying it into the commercial feed. White shrimp used were the size of 13.1 ± 0.06 grams in the amount of 10 shrimps per container. The size of the container was 50cm × 40cm × 40cm totaling 12 pieces (4 treatments and 3 replications). The treatments consisted of K (control), a bacterial dose of 104 CFU ml1, a bacterial dose of 106 CFU ml-1, and a bacterial dose of 10 8 CFU ml-1. The results showed that Bacillus coagulans as a probiotic feed was able to increase the nonspecific immunity of white shrimp. The treatment of Bacillus coagulans of 108 CFU ml-1 produced the best nonspecific immunity in white shrimp.