LB Broth-lyophilized Rabbit Anti-sheep Cell Haemolysin as a Simple Culture Medium for Cultivation of Leishmania Major Promastigotes

International Journal of Medical Laboratory Pub Date : 2018-11-21 DOI:10.18502/IJML.V6I2.1030

V. Nasiri, F. Jameie, H. Paykari

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Abstract

Background and Aims: The protozoan parasites of the genus Leishmania are the causative agents of various clinical diseases. Different methods of cultivation of Leishmania parasites are available. In the present study, the efficacy of the LB broth with rabbit lyophilized anti-sheep red blood cell haemolysin was evaluated in the cultivation of promastigotes of Leishmania major. Materials and Methods: Conventional LB broth medium was prepared and autoclaved for 15 min at 121°C and then lyophilized rabbit anti-sheep cell haemolysin was added at 1-10% final concentrations. The efficacy of the medium was evaluated by assessing the growth ability and replication patterns of the promastigotes of Leishmania major. Results: Medium with 1-10% lyophilized rabbit haemolysin supported the growth of the parasites and can be used for cultivation of Leishmania parasites with acceptable In vivo infectivity for research purpose. Conclusions: The ability of the parasites to survive and proliferate in the presence of lyophilized rabbit haemolysin indicates that this material is a good nutritional source. This study opens a new way to make low-cost medium that can be used in cultivation of Leishmania parasites

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LB肉汤冻干兔抗羊细胞溶血素简单培养基培养利什曼原虫

背景与目的:利什曼原虫属寄生虫是多种临床疾病的病原体。利什曼原虫有不同的培养方法。本研究评价了兔冻干LB肉汤抗羊红细胞溶血素对利什曼原虫原鞭毛菌培养的效果。材料和方法:制备常规LB肉汤培养基，在121℃下高压灭菌15 min，然后加入冻干的兔抗羊细胞溶血素，最终浓度为1-10%。通过观察利什曼原虫原殖体的生长能力和复制模式来评价培养基的效果。结果:含1-10%冻干溶血素的培养基支持利什曼原虫的生长，可用于利什曼原虫的培养，具有良好的体内感染性。结论:冻干兔溶血素对寄生虫的生存和增殖能力的影响表明该材料是一种良好的营养来源。本研究为制备利什曼原虫低成本培养基开辟了一条新途径

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International Journal of Medical Laboratory

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