Physicochemical Analysis, Elemental and Amino Acid Composition of Wool and Silk

{"title":"Physicochemical Analysis, Elemental and Amino Acid Composition of Wool and Silk","authors":"","doi":"10.33140/jgebr.04.02.15","DOIUrl":null,"url":null,"abstract":"The ash content of wool and silk was determined by burning the corresponding samples. The elemental composition of wool and silk was determined by ICP-mass spectral analysis, 26 macro- and microelements were found. Phosphorus has the maximum content in wool and silk samples, magnesium has the minimum content for wool, and potassium for silk. Chromato-mass spectrometry revealed 17 amino acids in wool and silk samples. Wool is characterized by a high content of phenylalanine, glycine, tyrosine, while silk is characterized by a high content of serine, glycine and threonine. Wool and silk proteins are also balanced in terms of nonessential amino acids. The analysis of the obtained IR spectra of proteins from wool and silk is carried out, absorption bands for the corresponding groups are marked. The corresponding vibrations of the amino groups of the carbonyl group are shown, which have an essential role in the structure of proteins.","PeriodicalId":235430,"journal":{"name":"Journal of Genetic Engineering and Biotechnology Research","volume":"43 10","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2022-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Genetic Engineering and Biotechnology Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33140/jgebr.04.02.15","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract

The ash content of wool and silk was determined by burning the corresponding samples. The elemental composition of wool and silk was determined by ICP-mass spectral analysis, 26 macro- and microelements were found. Phosphorus has the maximum content in wool and silk samples, magnesium has the minimum content for wool, and potassium for silk. Chromato-mass spectrometry revealed 17 amino acids in wool and silk samples. Wool is characterized by a high content of phenylalanine, glycine, tyrosine, while silk is characterized by a high content of serine, glycine and threonine. Wool and silk proteins are also balanced in terms of nonessential amino acids. The analysis of the obtained IR spectra of proteins from wool and silk is carried out, absorption bands for the corresponding groups are marked. The corresponding vibrations of the amino groups of the carbonyl group are shown, which have an essential role in the structure of proteins.
羊毛和丝绸的理化分析、元素和氨基酸组成
通过烧样法测定羊毛和丝绸的灰分含量。用icp -质谱法测定了羊毛和蚕丝的元素组成,发现了26种宏、微量元素。羊毛和丝绸样品中磷含量最高,羊毛样品中镁含量最低,丝绸样品中钾含量最低。质谱分析发现羊毛和丝绸样品中含有17种氨基酸。羊毛的特点是苯丙氨酸、甘氨酸、酪氨酸含量高,而丝绸的特点是丝氨酸、甘氨酸和苏氨酸含量高。羊毛和丝绸蛋白在非必需氨基酸方面也很平衡。对羊毛和丝绸中蛋白质的红外光谱进行了分析,标记了相应基团的吸收带。显示了羰基的氨基的相应振动,这在蛋白质结构中起着重要作用。
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