Review on microbial degradation of zearalenone and aflatoxins

Q2 Agricultural and Biological Sciences

Grain Oil Science and Technology Pub Date : 2020-09-01 DOI:10.1016/j.gaost.2020.05.002

Jiaxing Wang , Yanli Xie

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引用次数: 25

Abstract

The widespread contamination by mycotoxins, especially zearalenone and aflatoxins, in crops and their by-products has caused severe undesirable effects on human health and commercial trade. Researchers had screened out microorganisms from various media for degrading zearalenone and aflatoxins, and the results of a lot of studies showed that enzymes derived from microbial strains play a key role in degrading mycotoxins. Genetic engineering technology had been applied to improve the heterologously expressed degrading enzymes in several mature microbial hosts such as Escherichia coli and Pichia pastoris. The separated and purified recombinant enzyme had high activity in degrading mycotoxins in vitro. This review summarized the types of mycotoxins-degrading microorganisms and enzymes, and the progress on synthesis of heterologously expressed degrading enzymes by genetic engineering technology as well as related researches on improving the effect of degrading enzymes. We also prospected the future development in the study of using recombinant enzymes formed by genetic engineering technology to realize the simultaneous degradation of multiple mycotoxins in crops.

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玉米赤霉烯酮和黄曲霉毒素的微生物降解研究进展

真菌毒素，特别是玉米赤霉烯酮和黄曲霉毒素在作物及其副产品中的广泛污染，对人类健康和商业贸易造成了严重的不良影响。研究人员从各种培养基中筛选出了降解玉米赤霉烯酮和黄曲霉毒素的微生物，大量研究结果表明，来自微生物菌株的酶在降解真菌毒素中起着关键作用。利用基因工程技术改进了大肠杆菌和毕赤酵母等成熟微生物宿主中异源表达的降解酶。分离纯化的重组酶在体外具有较高的真菌毒素降解活性。本文综述了真菌毒素降解微生物和酶的类型，利用基因工程技术合成异源表达的降解酶的研究进展以及提高降解酶效果的相关研究进展。展望了利用基因工程技术形成的重组酶实现作物中多种真菌毒素同时降解的研究前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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