Natural lignin modulators improve bagasse saccharification of sugarcane and energy cane in field trials

IF 5.9 3区 工程技术 Q1 AGRONOMY
Viviane Fátima de Oliveira, Lucas Figueiredo, Gabriel de Oliveira Correia, Maria de Fátima Pires da Silva Machado, Hugo Zeni Neto, Wanderley Dantas dos Santos, Claudete Aparecida Mangolin
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引用次数: 0

Abstract

The burgeoning cellulosic ethanol industry necessitates advancements in enzymatic saccharification, effective pretreatments for lignin removal, and the cultivation of crops more amenable to saccharification. Studies have demonstrated that natural inhibitors of lignin biosynthesis can enhance the saccharification of lignocellulose, even in tissues generated several months post-treatment. In this study, we applied daidzin (a competitive inhibitor of coniferaldehyde dehydrogenase), piperonylic acid (a quasi-irreversible inhibitor of cinnamate 4-hydroxylase), and methylenedioxy cinnamic acid (a competitive inhibitor of 4-coenzyme A ligase) to 60-day-old crops of two conventional Brazilian sugarcane cultivars and two energy cane clones, bred specifically for enhanced biomass production. The resultant biomasses were evaluated for lignin content and enzymatic saccharification efficiency without additional lignin-removal pretreatments. The treatments amplified the production of fermentable sugars in both the sugarcane cultivars and energy cane clones. The most successful results softened the most recalcitrant lignocellulose to the level of the least recalcitrant of the biomasses tested. Interestingly, the softest material became even more susceptible to saccharification.

Abstract Image

天然木质素调节剂改善蔗渣糖化甘蔗和能源甘蔗的田间试验
蓬勃发展的纤维素乙醇工业需要在酶解糖化、有效的预处理木质素去除和更适合糖化的作物种植方面取得进展。研究表明,木质素生物合成的天然抑制剂可以增强木质素纤维素的糖化,即使在处理后几个月产生的组织中也是如此。在这项研究中,我们将大豆苷(一种松柏醛脱氢酶的竞争性抑制剂)、胡椒酰酸(一种肉桂酸4-羟化酶的准不可逆抑制剂)和亚二氧基肉桂酸(一种4-辅酶a连接酶的竞争性抑制剂)施用于两个巴西传统甘蔗品种和两个专门为提高生物质产量而培育的能源甘蔗无克隆的60天龄作物上。在不进行除木质素预处理的情况下,对所得生物质的木质素含量和酶解糖化效率进行了评价。这些处理增加了甘蔗品种和能量甘蔗无性系可发酵糖的产量。最成功的结果是将最顽固的木质纤维素软化到所测生物质中最不顽固的水平。有趣的是,最软的物质更容易被糖化。
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来源期刊
Global Change Biology Bioenergy
Global Change Biology Bioenergy AGRONOMY-ENERGY & FUELS
CiteScore
10.30
自引率
7.10%
发文量
96
审稿时长
1.5 months
期刊介绍: GCB Bioenergy is an international journal publishing original research papers, review articles and commentaries that promote understanding of the interface between biological and environmental sciences and the production of fuels directly from plants, algae and waste. The scope of the journal extends to areas outside of biology to policy forum, socioeconomic analyses, technoeconomic analyses and systems analysis. Papers do not need a global change component for consideration for publication, it is viewed as implicit that most bioenergy will be beneficial in avoiding at least a part of the fossil fuel energy that would otherwise be used. Key areas covered by the journal: Bioenergy feedstock and bio-oil production: energy crops and algae their management,, genomics, genetic improvements, planting, harvesting, storage, transportation, integrated logistics, production modeling, composition and its modification, pests, diseases and weeds of feedstocks. Manuscripts concerning alternative energy based on biological mimicry are also encouraged (e.g. artificial photosynthesis). Biological Residues/Co-products: from agricultural production, forestry and plantations (stover, sugar, bio-plastics, etc.), algae processing industries, and municipal sources (MSW). Bioenergy and the Environment: ecosystem services, carbon mitigation, land use change, life cycle assessment, energy and greenhouse gas balances, water use, water quality, assessment of sustainability, and biodiversity issues. Bioenergy Socioeconomics: examining the economic viability or social acceptability of crops, crops systems and their processing, including genetically modified organisms [GMOs], health impacts of bioenergy systems. Bioenergy Policy: legislative developments affecting biofuels and bioenergy. Bioenergy Systems Analysis: examining biological developments in a whole systems context.
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